Manipal Journal of Science and Technology
Abstract
Respiratory infection caused by the bacterial species is a leading cause of morbidity and mortality globally, especially in immunocompromised people. All available diagnostic techniques for pneumonia detection are relatively limited due to time-consuming stages, selectivity, and the need for invasive procedures. To overcome the current challenges, we developed an analytical technique, utilizing a carbon mesh-supported thin film solid-phase microextraction (TF-SPME) technique coupled to gas chromatography-mass spectrometry for monitoring of pathogen-specific metabolites from bacterial culture media of E. coli bacterial strain. We exposed the TF-SPME device from the headspace of the culture media of the bacteria and extracted the metabolites during the growth phase of the E. coli. TF-SPME was thermally desorbed in GC-MS to identify the compounds. We observed the pathogen-emitted metabolites, including Pentane, 3-methyl (RT 1.645), Cyclopentane, methyl (RT 1.876), Cyclohexane (RT 2.099), 2-pentanone (RT 2.3), Benzene, 1-ethyl-4-methyl (RT 5.673), and Cyclopropane, 1, 1-dichloro-3 (1, 1-dimethyl ethyl)-2, 2-dimethyl (RT 12.525) in culture media. This technique may be helpful for the non-invasive diagnosis of bacterial pathogens without culture studies.
Recommended Citation
S, Keerthana; Illanad, Gouri; and Ghosh, Chiranjit Dr.
(2023)
"Characterization of Pathogen-specific Metabolites by Thin-film Solid Phase Microextraction Analytical Tool,"
Manipal Journal of Science and Technology: Vol. 8:
Iss.
2, Article 4.
Available at:
https://impressions.manipal.edu/mjst/vol8/iss2/4