Document Type

News Article

Abstract

Extracellular vesicles, including exosomes and microvesicles, offer great opportunities for understanding biological mechanisms and diagnostic prospects for various disorders. Our laboratory explores the potential of extracellular vesicles from multiple sources for mechanistic studies and for therapeutic applications to various neurological disorders. The laboratory has a well-established facility for generating, isolating and characterizing extracellular vesicles and aims to bring these EVs to clinical applications.

The pathological alterations that manifest during early embryonic development due to inherited and acquired factors trigger various neurodevelopmental disorders, including epilepsy, neuronal migration disorders and neurocognitive dysfunctions. Our work also focuses on the potential use of stem cells and rodent models for elucidating neurodevelopmental disorders related to interneuron development and dysfunction. The laboratory has a well-established facility for stem cell research. It utilizes established directed differentiation protocols for generating multiple types of human neurons and 3D organoids from pluripotent stem cells.

We utilize fluorescence microscopy, dual and triple immunofluorescence, immunohistochemistry, western blot, qPCR, ELISA, and other biochemical and cell-based assays, etc. in the facility. At the same time, gene editing, RNA sequencing, and proteomic and lipidomic studies are being performed through collaborative research. The laboratory depends on Kasturba Hospital Manipal for the clinical data and the central animal facility for animal studies.

Recent publications

  1. https://pubmed.ncbi.nlm.nih.gov/35915118
  2. https://pubmed.ncbi.nlm.nih.gov/33370574
  3. https://pubmed.ncbi.nlm.nih.gov/30559206/
  4. https://pubmed.ncbi.nlm.nih.gov/28396435/
  5. https://pubmed.ncbi.nlm.nih.gov/35035379/

Websites:

https://www.dineshupadhyalab.com/team

https://scholar.google.com/citations?hl=en&user=qvVv52oAAAAJ&view_op=listworks&sortby=pubdate

Publication Date

Winter 11-1-2022

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