Surveillance of influenza A and B viruses from community and hospital wastewater treatment plants
Document Type
Article
Publication Title
Environmental Microbiology Reports
Abstract
Influenza virus is a well-known pathogen that can cause epidemics and pandemics. Several surveillance methods are being followed to monitor the transmission patterns and spread of influenza in the community. Wastewater-based Epidemiology (WBE) can serve as an additional tool to detect the presence of influenza viruses. The current study primarily focuses on surveillance of Influenza A and Influenza B in wastewater treatment plant (WWTP) samples. A total of 100 wastewater samples were collected in July (n = 50) and August (n = 50) 2023 from four different WWTPs in Manipal and Udupi, district of Karnataka, India. The WWTP samples were processed and tested by Real-Time reverse transcriptase PCR (RT-PCR). The data generated was analysed in comparison with the clinical Influenza cases. Of the 100 samples, 18 (18%) tested positive for Influenza A virus and 2 (2%) tested positive for Influenza B virus, with a viral load ranging 1.4 x 102–2.2 x 103 gc/L for influenza A virus and 5.2 x 103–7.7 x 103gc/L for influenza B virus. On correlating the WWTP positivity with clinical case, it was found that influenza clinical cases and virus positivity in wastewater increased simultaneously, emphasizing WBE as a concurrent method for monitoring influenza virus activity.
DOI
10.1111/1758-2229.13317
Publication Date
8-1-2024
Recommended Citation
Panneerselvam, Sneka; Manayan Parambil, Athira; Jayaram, Anup; and Varamballi, Prasad, "Surveillance of influenza A and B viruses from community and hospital wastewater treatment plants" (2024). Open Access archive. 10186.
https://impressions.manipal.edu/open-access-archive/10186
