"Human Eye Lens Fluid Analysis Using High-Performance Liquid Chromatogr" by Sulatha V. Bhandary, Sphurti S. Adigal et al.
 

Human Eye Lens Fluid Analysis Using High-Performance Liquid Chromatography-LED-Induced Fluorescence to Explore Cataract: A Pilot Study

Document Type

Article

Publication Title

Journal of Biomedical Photonics and Engineering

Abstract

Cataract is the common cause of reversible blindness and visual impairment. It is common in low socioeconomic groups in developing countries. This paper describes the application of an assembled High-Performance Liquid Chromatography-LED-Induced Fluorescence (HPLC-LED-IF) system to study the protein profiles of lens fluid samples from cataract patients with and without diabetes. The HPLC-LED-IF system assembled in our laboratory was used to record protein profiles of lens fluid samples obtained from volunteers having cataract with diabetes and without diabetes who have undergone cataract surgery. Statistical analyses such as Descriptive Statistics and Principal Component Analysis (PCA) were conducted to observe any kind of changes in the protein pattern of the two classes of samples. High-Performance Liquid Chromatography combined with LED-Induced Fluorescence (HPLC-LIF) generated lens fluid protein profile data of the two categories (cataract with and without diabetes) of samples have shown considerable changes in their pattern. These changes are confirmed by Descriptive Statistics and PCA. Protein profiles of lens fluid using an assembled HPLC-LED-IF system have shown significant differences in relative intensities of crystalline proteins of cataract with diabetes and without diabetes. UV absorption and fluorescence spectroscopy of the lens fluid have shown valuable information on the absorption and fluorescence intensity variations. The origin of the changes has to be further studied to get more information on this observation. © 2024 Journal of Biomedical Photonics & Engineering.

DOI

10.18287/JBPE24.10.030302

Publication Date

1-1-2024

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