A pilot study on comparative analysis of minimum inhibitory concentration and mutant prevention concentration of conjunctival bacterial isolates against fluoroquinolones
Document Type
Article
Publication Title
Indian Journal of Microbiology Research
Abstract
Background: The aim of this study was to compare the Minimum Inhibitory Concentration (MIC) and Mutant Prevention Concentration (MPC) of fluoroquinolones, including ciprofloxacin, moxifloxacin, and gatifloxacin, against Staphylococcus aureus and coagulase-negative Staphylococci (CONS) isolated from conjunctival swabs. Materials and Methods: 25 isolates of Staphylococcus spp., obtained from conjunctival swabs submitted to the Department of Microbiology, Vision Research Foundation, Sankara Nethralaya, were included in this study. The identification and confirmation of Staphylococcus spp. were performed using standard microbiological techniques. The MIC and MPC were determined using the agar dilution method, following protocols from previous studies. The MIC50, MIC90, MPC50, and MPC90 values for the above three fluoroquinolones were calculated and analysed. Results: Out of all 25 isolates, 20 were CONS and 5 were Staphylococcus aureus. In our study, gatifloxacin had least MIC and MPC values when compared to ciprofloxacin and moxifloxacin of gatifloxacin had lower MPC50 and MPC90 values in comparison to ciprofloxacin and moxifloxacin. Our study shows that Gatifloxacin had least MIC and MPC values when compared to ciprofloxacin and moxifloxacin. Besides, MPC of ciprofloxacin, moxifloxacin and gatifloxacin showed wider range of distribution than the MIC. Conclusion: Gatifloxacin demonstrated effective inhibition of resistant mutant strains at lower concentrations compared to ciprofloxacin and moxifloxacin. Additionally, future studies with a larger number of isolates, incorporating pharmacokinetic and pharmacodynamic parameters, will provide essential information on therapeutic outcomes and resistance prevention.
First Page
180
Last Page
185
DOI
10.18231/j.ijmr.2024.033
Publication Date
1-1-2024
Recommended Citation
Selvi, Krishnan; Kavitha, A. V.; Anand, Sunderesan; and Parthasarathy, Durgadevi, "A pilot study on comparative analysis of minimum inhibitory concentration and mutant prevention concentration of conjunctival bacterial isolates against fluoroquinolones" (2024). Open Access archive. 10675.
https://impressions.manipal.edu/open-access-archive/10675
