Acute Toxicity Study and Antidiabetic Activity of Marine alga-Halimeda gracilis Chooranam (HGC) in Freshwater Zebrafish Model

Document Type

Article

Publication Title

Biomedical and Pharmacology Journal

Abstract

The study aimed to assess the acute toxicity and anti-diabetic activity of Halimeda gracilis (green marine alga). The Halimeda gracilis were collected from the coastal area of the Gulf of Mannar biosphere reserve and shade dried. Methanolic extract of Halimeda gracilis (MEHG) was prepared and it was screened for acute toxicity and anti-diabetic activity in the Zebrafish model. In the Acute toxicity study, the Zebrafishes were grouped into 6 groups and dosed with 6.25, 12.5, 25, 50, and 100mg/L of MEHG and observed at 0, 24, 48, 72, and 96 hours’ intervals. For anti-diabetic activity analysis diabetes was induced using streptozotocin (STZ). The Zebrafish were divided into six groups- control group, positive control, diabetic Zebrafish with three doses of MEHG, and standard control (treated with metformin). Acute toxicity study showed no significant behavioral changes and LC50 was determined as 100mg/L. In the diabetic study, test groups when compared to the control group showed: a significant reduction in both fasting and postprandial blood glucose levels and significant changes in the regeneration of pancreatic â-cells, and reduced vacuolization in the islets of Langerhans. Images of the regenerating caudal fins taken at 24, 48 and 72-hours post-amputation displayed significant limb regeneration in MEHG treated fish compared to the control group. These results prove that MEHG in STZ- induced diabetic Zebrafish possess potent anti-diabetic action by ameliorating blood glucose regulation, promoting pancreatic cell regeneration, minimizing long-term diabetic complications by preventing the emergence of metabolic memory but no behavioral changes.

First Page

1647

Last Page

1653

DOI

10.13005/bpj/2265

Publication Date

1-1-2021

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