Blue LED light exposure induces metabolic rewiring in vitreous tissues in rat models

Document Type

Article

Publication Title

Journal of King Saud University - Science

Abstract

Blue light exposure induced retinal damage has been extensively studied. Retinal damages are closely associated with cellular and biochemical changes occurring in the vitreous and hence, understanding metabolic changes in vitreous tissue might serve as clinical importance to indicate ocular/vision health. In the present study, we have investigated the influence of blue light-emitting diodes (LED) on the vitreous metabolome and further, we show amelioration of altered metabolite levels upon blue light blocking lenses (BBLs). A total of n = 24 (n = 6 in each group; control, light exposure without lenses, two different BBLs) male Wistar rats were subjected to blue light exposure (LEDs, 450–500 lx) without or with BBLs (400–490 nm) for 28 days on a 12:12 h light–dark cycle. Post-exposure the vitreous fluid aspirated was subjected for untargeted liquid chromatography/mass spectrometry-based metabolomics analysis. Analyzed vitreous revealed blue light significantly modulated metabolites such as Tyrosine, L-Histidine (p = 0.02), L-Isoleucine (p = 0.007), Valine (p = 0.04) and D-Proline (P < 0.001) along with affected homocysteine degradation and nitric oxide signalling pathway. Our findings suggest exposure to blue LED poses a significant hazard to the vitreous as it alters the vitreous metabolites, and it is partially ameliorated by commercially available BBLs.

DOI

10.1016/j.jksus.2022.101986

Publication Date

6-1-2022

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