Bioanalytical RP-HPLC method validation for resveratrol and its application to pharmacokinetic and drug distribution studies

Document Type

Article

Publication Title

Journal of Applied Pharmaceutical Science

Abstract

In the present study, an attempt was made to develop and validate a highly sensitive reverse phase-High Pressure Liquid Chromatography (HPLC) method for the quantification of resveratrol (RVT) in the plasma and tissue matrix. Pharmacokinetics and tissue distribution studies were carried out in rats after oral administration of RVT. RVT was eluted using 0.1% orthophosphoric acid and acetonitrile as the mobile phase. The developed method showed good recovery and linearity without any interference from the biological matrix. From the pharmacokinetic studies, the plasma half-life of RVT was found to be 4.4 ± 0.12 hours. It was observed from bio-distribution studies that RVT preferentially accumulated in the high perfused organs. The developed method was successfully applied for the quantification of RVT in different biological matrices

First Page

158

Last Page

164

DOI

10.7324/JAPS.2021.120216

Publication Date

2-1-2022

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