Protocol for isolation of mouse pre-implantation embryos for gene expression analysis

Document Type

Article

Publication Title

STAR Protocols

Abstract

Visualizing and quantifying the numerous factors that regulate murine pre-implantation embryonic development is technically challenging. Here, we present a protocol for the isolation of pre-implantation embryos at multiple stages of embryonic development to study gene expression. We describe steps for isolating RNA and cDNA synthesis from a small number of embryos. We then detail an immunofluorescence assay for the detection and localization of protein of interest by confocal microscopy in the pre-implantation embryos. For complete details on the use and execution of this protocol, please refer to Varghese et al.1

DOI

10.1016/j.xpro.2023.102479

Publication Date

9-15-2023

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