Antibiogram of Enterococcus spp. associated with post-operative wound infections

Document Type

Article

Publication Title

Infectious Diseases: News, Opinions, Training

Abstract

Multiple drug resistant enterococci with resistance to high level aminoglycoside, β-lactums, and glycopeptide are increasingly being isolated from pus samples of hospitalised individuals. Risk causes include an immunocompromised state of the host, a prolonged hospital stay, and treatment with broad spectrum antibiotics like cephalosporins and vancomycin. Aim: to study the antimicrobial susceptibility patterns and hemolysin production by various Enterococcus spp. isolated from infected surgical site wounds. Material and methods. A total of 30 Enterococcus spp. isolated from post-surgery wound infections were included in the study. Standard bio-chemical tests identified and speciated them. The production of hemolysin by isolates was detected by inoculation of Enterococcus spp. on 5% sheep blood agar plate. To perform antibiotic sensitivity and interpret the results as per CLSI guidelines Kirby–Bauer’s disc diffusion test was used. The Vancomycin Minimum inhibitory concentration (MIC) was detected by agar dilution method. Results and discussion. Among the 30 enterococcal isolates, 18 (60%) were identified as E. faecalis, 4 (13%) were E. faecium, and E. dispar, 2 (7%) each were E. pseudoavium and E. durans. 11 (61%) E. faecalis and 2 (7%) E. faecium were haemolysin positive. 4 (22%) E. faecalis were resistant to High Level Streptomycin. 8 (44%) E. faecalis and 1 (25%) E. faecium showed resistance to High Level Gentamicin. All Enterococcus isolates were susceptible to vancomycin by disc diffusion and agar dilution methods with an MIC of ≤2 μg/mL. Conclusion. Effective and appropriate identification and recognition of drug resistant Enterococcus spp. help in minimizing the morbidity and mortality in hospitalized patients and limit the spread of drug resistance in hospitals.

First Page

84

Last Page

89

DOI

10.33029/2305-3496-2023-12-1-84-89

Publication Date

1-1-2023

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