Summary of - Rodent models for dry eye syndrome: Standardization using benzalkonium chloride and scopolamine hydrobromide
Document Type
Article
Abstract
Study Background: Dry eye disease is a highly prevalent ocular condition that significantly affects the quality of life and presents a major challenge in ophthalmology. Animal models play a crucial role in investigating pathophysiology and developing effective treatments.
Research Goals and Hypotheses: The goal of this study was to compare and standardize two dry eye disease rodent models and explore their recovery aspects.
Methodological Approach: In this study, we have standardized benzalkonium chloride and scopolamine-induced dry eye disease models which represent two different classes of dry eye i.e., evaporative dry eye and aqueous deficient dry eye, respectively. After the development of dry eye conditions, a self-recovery period of seven days was granted to assess the reversal of the induced changes. The dry eye condition was assessed by measuring tear volume, corneal slit lamp imaging, and histological examination of the cornea, the lacrimal, and the harderian gland.
Results and Discoveries: The study indicated the development of chronic inflammation of the cornea and lacrimal gland in the case of benzalkonium after five days of the treatment, while the scopolamine-treated group showed chronic inflammation of the lacrimal gland after five days and corneal inflammation after seven days of administration. The recovery study suggested that after discontinuation of the inducing agent, the dry eye symptoms were still persistent suggesting the utility of the model in evaluating dry eye treatments. The study highlights the comparative changes in both models along with recovery which can serve as a base for drug discovery and development against dry eye disease.
Publication Date:
2023
Recommended Citation
Chaudhari P, Satarker S, Thomas R, Theruveethi N, Ghate V, Nampoothiri M, Lewis SA, Rodent models for dry eye syndrome: Standardization using benzalkonium chloride and scopolamine hydrobromide, Life Sciences, Volume 317,2023,121463,ISSN 0024-3205
Publication Date
2023
Recommended Citation
Chaudhari, Pinal; Satarker, Sairaj; Thomas, Rinu; Theruveethi, Nagarajan; Ghate, Vivek; Nampoothiri, Madhavan; and Lewis, Shaila A., "Summary of - Rodent models for dry eye syndrome: Standardization using benzalkonium chloride and scopolamine hydrobromide" (2023). Open Access archive. 9262.
https://impressions.manipal.edu/open-access-archive/9262
