Summary of - How can omics inform diabetic foot ulcer clinical management? A whole genome comparison of four clinical strains of Staphylococcus aureus

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Article

Abstract

The research article titled “How can omics inform diabetic foot ulcer clinical management? A whole genome comparison of four clinical strains of Staphylococcus aureus” by Dr. Tanwar AS, Ms. Shruptha P, Dr. Paul B, Dr. Murali TS, Dr. Brand A, and Dr. Satyamoorthy K focusses on whole genome comparison of four clinical strains of S. aureus obtained from diabetic foot ulcer. Management of chronic, non-healing foot ulcers is a major challenge due to the presence of microbial communities at the infection site. It has been shown that biofilm-forming S. aureus are capable of specifically inhibiting wound healing and worsening the wound infection. In the current study, whole-genome comparative analysis of four S. aureus strains (MUF168, MUF256, MUM270, and MUM475) isolated from DFU patients was performed to understand their antimicrobial resistance (AMR) and virulence profiles, potential drug targets and degree of sequence variation.

KEY FINDINGS:

  • The draft genome of MUF168, MUF256, MUM270, and MUM475 strains consisted of 59 (2.75 mb), 58 (2.78 mb), 125 (2.83 mb), and 74 (2.85 mb) contigs, respectively.
  • Several genes were categorized in ‘‘function unknown’’ category while many hypothetical genes were identified by RAST indicating considerable amount of S. aureus genome function is unknown and remains to be explored.
  • A total of 34 different AMR genes conferring resistance to 23 antibiotics were predicted to be present in the 4 S. aureus strains and these results were validated by PCR.
  • A total of 76 virulence factors were identified across 4 strains
  • Fibronectin-binding proteins fnbA and fnbB that play an important role in host cell attachment were found in all four strains
  • A total of 56 toxin genes were present in all the 4 strains
  • A total of 16 drug targets were found in the DrugBank database of which 15 showed significant sequence similarity to sequence contigs of 4 strains

Citation to the base paper: Tanwar AS, Shruptha P, Paul B, Murali TS, Brand A, Satyamoorthy K. 2023. How can omics inform diabetic foot ulcer clinical management? A whole genome comparison of four clinical strains of Staphylococcus aureus. OMICS: A Journal of Integrative Biology 27, 51-61.

Publication Date

2023

Recommended Citation

Tanwar AS, Shruptha P, Paul B, Murali TS, Brand A, Satyamoorthy K., " How can omics inform diabetic foot ulcer clinical management? A whole genome comparison of four clinical strains of Staphylococcus aureus" (2023). Open Access archive.

Publication Date

2023

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