Summary of - Next-Generation Sequencing technology for the diagnosis of microbial infections in hard-to-heal wounds

Document Type

Article

Abstract

The review article titled “Next-Generation Sequencing technology for the diagnosis of microbial infections in hard-to-heal wounds” by Ms. Nayak N, Dr. Satyamoorthy K, and Dr. Murali TS. focusses on Next-Generation Sequencing (NGS) approaches to study wound microbiome. In this study, we highlight the advantages and disadvantages associated with traditional and advanced molecular technologies, such as NGS, to study the wound-associated microbial communities. Hard-to-heal wounds associated with diabetic foot ulcers are a significant cause of morbidity and mortality, while microbial infections further delay the healing process. Whild traditional culture-based methods are widely studied to understand and characterize microbial communities within the hard-to-heal wound, it comes with its own limitations. This method underestimates or excludes most of the dominant species while being oversensitive towards others. NGS based methods often rely on sequencing of genes coding for small subunit ribosomal RNA and internal transcribed spacer locus for identification of bacteria and fungi, respectively. They provide more quantitative data in a faster, more cost-effective manner. A full understanding of the complete diversity of the wound microbiome will help in devising effective treatment regimens for hard-to-heal wounds.

Citation to the base paper: Nayak N, Satyamoorthy K, Murali TS. 2023. Next-Generation Sequencing technology for the diagnosis of microbial infections in hard-to-heal wounds. Journal of Wound Care 32, xcvii-cix.

Publication Date

2023

Recommended Citation

Nayak N, Satyamoorthy K, Murali TS, " Next-Generation Sequencing technology for the diagnosis of microbial infections in hard-to-heal wounds" (2023). Open Access archive. xxx. https://impressions.manipal.edu/open-access-archive/xxx

Publication Date

2023

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