Diversity of post-translational modifications and cell signaling revealed by single cell and single organelle mass spectrometry
Document Type
Article
Publication Title
Communications Biology
Abstract
The rapid evolution of mass spectrometry-based single-cell proteomics now enables the cataloging of several thousand proteins from single cells. We investigated whether we could discover cellular heterogeneity beyond proteome, encompassing post-translational modifications (PTM), protein-protein interaction, and variants. By optimizing the mass spectrometry data interpretation strategy to enable the detection of PTMs and variants, we have generated a high-definition dataset of single-cell and nuclear proteomic-states. The data demonstrate the heterogeneity of cell-states and signaling dependencies at the single-cell level and reveal epigenetic drug-induced changes in single nuclei. This approach enables the exploration of previously uncharted single-cell and organellar proteomes revealing molecular characteristics that are inaccessible through RNA profiling.
DOI
10.1038/s42003-024-06579-7
Publication Date
12-1-2024
Recommended Citation
Mun, Dong Gi; Bhat, Firdous A.; Joshi, Neha; and Sandoval, Leticia, "Diversity of post-translational modifications and cell signaling revealed by single cell and single organelle mass spectrometry" (2024). Open Access archive. 9797.
https://impressions.manipal.edu/open-access-archive/9797
