Summary of - Bisphenol A exposure modulates reproductive and endocrine system, mitochondrial function and cellular senescence in female adult rats: A hallmarks of polycystic ovarian syndrome phenotype

Document Type

Article

Abstract

Study Background: Endocrine disrupting chemical Bisphenol A (BPA) mimics the estrogen, potentially damaging female reproductive system. The increased BPA exposure in humans leads to adverse reproductive health outcomes, including male sexual dysfunction, reduced fertility, endometriosis, and polycystic ovarian syndrome (PCOS).

Aim of the study: The study aimed to examine the molecular pathomechanisms in the BPA-treated PCOS rat model that closely mimics the PCOS’s reproductive, hormonal, ovarian, mitochondrial, and senescence disturbances in humans.

Methods:

  • Different doses of BPA (low, high) were administered on Sprague Dawley (SD) female rats for 90 days with ethical clearance from IAEC committee, MAHE, Manipal
  • Evaluation of estrous cycle staging from 75th day to 90th day of BPA treatment in SD rats
  • Ovaries were collected and histopathology was performed
  • RNA extraction, cDNA synthesis and RT-PCR was performed to evaluate the target genes involved in ovarian steroidogenesis
  • Estimation of reactive oxygen species, cellular senescence was performed in ovarian tissues and immunofluorescence staining for CDKN2A in ovarian tissues
  • Statistical analysis was performed to find significant variants between the study groups

Results and Discussion

  • No significant differences in serum testosterone, estradiol, and progesterone levels observed between control, vehicle control, LD, and HD rats.
  • BPA altered expression patterns of key enzymes of steroidogenesis, regulating ovarian steroidogenesis.
  • Elevated ROS production upon BPA exposure in dose-dependent manner, with higher mean intensity in HD rats and LD rats compared to the VC group.
  • CDKN2A protein level in ovaries reported maximum mean intensity in HD rats compared to vehicle control animals.

Conclusion

The study provides an insight into BPA's effects on ovulatory, hormonal, mitochondrial dysfunction, and senescence.

Citation to the base paper: Prabhu, N. B., Adiga, D., Kabekkodu, S. P., Bhat, S. K., Satyamoorthy, K., & Rai, P. S. (2022). Bisphenol A exposure modulates reproductive and endocrine system, mitochondrial function and cellular senescence in female adult rats: a hallmarks of polycystic ovarian syndrome phenotype. Environmental Toxicology and Pharmacology, 96, 104010.

Publication Date

2022

Publication Date

2022

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